induro rt reaction buffer (New England Biolabs)

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New England Biolabs induro rt reaction buffer
Induro Rt Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/induro+rt+reaction+buffer/Induro+Reverse+Transcriptase/pmc12848939-153-2-6
Average 98 stars, based on 135 article reviews

induro rt reaction buffer - by Bioz Stars, 2026-10

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Article Title: Sni445 recruits box C/D snoRNPs snR4 and snR45 to guide ribosomal RNA acetylation by Kre33
Article Snippet: For reverse transcription, 200 pg of RNA from individual treatment reactions were incubated with 4 pmol of the respective primers [18S_h34_rev (5′-GGTTAAGGTCTCGTTCGTTATCG-3′) for analysis of the acetylated cytidine in helix h34, or 18S_h45_rev (5′-TAATGATCCTTCCGCAGGTTCACCTAC-3′) for analysis of the acetylated cytidine in helix h45], 10 nmol dNTP mix (NEB), and nuclease-free water in a final reaction volume of 10 μl, incubated at 65°C for 5 min, and promptly transferred on ice. .. Then 1× Induro RT Reaction Buffer (NEB), 8 U of murine RNase inhibitor (NEB), and 200 U of Induro Reverse Transcriptase (NEB) were added in a final volume of 20 μl. .. Samples were incubated at 55°C for 10 min, followed by inactivation at 95°C for 1 min. A 2 μl aliquot of the cDNA products was used as template in a 50 μl PCR with 1× Q5 reaction buffer (NEB), 25 pmol of forward primers [18S_h34_fwd (5′-AAGGAATTGACGGAAGGGC-3′) or 18S_h45_fwd (5′-CGTCGCTAGTACCGATTGAATGGCTTAG-3′)] and 25 pmol of the respective reverse primers used for the reverse transcription (18S_h34_rev or 18S_h45_rev, respectively), 10 nmol dNTP mix, and 2.5 U of Q5 Polymerase (NEB).

Article Title: Epstein-Barr Virus Encoded lncRNAs Control the Viral Lytic Switch
Article Snippet: .. For each amplicon a reverse transcription (RT) reaction was prepared by mixing 1ug of RNA, 0.5ul of Single-stranded Binding (SSB) protein, 4ul of 10mM reverse primer, 2ul of 10mM dNTPs, 0.4ul of RNase Inhibitor, 8ul of Induro RT reaction buffer (NEB), 2ul of Induro Reverse transcriptase (NEB), and completed to a final volume of 40ul with RNase-free water. ..

Article Title: Sni445 recruits box C/D snoRNPs snR4 and snR45 to guide ribosomal RNA acetylation by Kre33.
Article Snippet: For reverse transcription, 200 pg of RNA from individual treatment reactions were incubated with 4 pmol of the respective primers [18S_h34_rev (5 ′ - GGTT AAGGTCTCGTTCGTT A TCG-3 ′ ) for analysis of the acetylated cytidine in helix h34, or 18S_h45_rev (5 ′ - T AA TGA TCCTTCCGC AGGTTC ACCTAC-3 ′ ) for analysis of the acetylated cytidine in helix h45], 10 nmol dNTP mix (NEB), and nuclease-free water in a final reaction volume of 10 μl, incubated at 65 ◦C for 5 min, and promptly transferred on ice. .. Then 1 × Induro RT Reaction Buffer (NEB), 8 U of murine RNase inhibitor (NEB), and 200 U of Induro Reverse Transcriptase (NEB) were added in a final volume of 20 μl. .. Samples were incubated at 55 ◦C for 10 min, followed by inactivation at 95 ◦C for 1 min. A 2 μl aliquot of the cDNA products was used as template in a 50 μl PCR with 1 × Q5 reaction buffer (NEB), 25 pmol of forward primers [18S_h34_fwd (5 ′ - AA GGAATTGA CGGAA GGGC-3 ′ ) or 18S_h45_fwd (5 ′ - CGTCGCT AGT ACCGA TTGAA TGGCTT AG-3 ′ )] and 25 pmol of the respective reverse primers used for the reverse transcription (18S_h34_rev or 18S_h45_rev, respectively), 10 nmol dNTP mix, and 2.5 U of Q5 Polymerase (NEB).

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